OPTIPREP™ APPLICATION SHEET INDEX
EUKARYOTIC AND PROKARYOTIC CELLS

• The Index is divided into two sections:

A. General methods for preparing and analysing gradients
B. An alphabetical cell type index. Commonly isolated cells such as mononuclear cells are often further categorized into species or tissue source. If a cell type is not in this index, it may be necessary to develop a customized method: in Section A see “Fractionation of a mixed population of cells” for some guidance.

• Click on the relevant [Application Sheet] for a detailed protocol. In some cases more than one Application Sheet for a specific cell type may be provided if different practical strategies are available.

A. GENERAL METHODOLOGY

Preparation of gradient solutions [Application Sheet C01]
Preparation of gradients [Application Sheet C02]
Fractionation of a mixed population of cells [Application Sheet C15]
Analysis of gradients [Application Sheet C51]

B. ALPHABETICAL CELL INDEX

Alveolar cells (see “Pulmonary cells”)
Bacteria
Anaplasma phagocytophilum [Application Sheet C49]
Chlamydophila abortus [Application Sheet C49]
Cyanobacteria [Application Sheet C49]
Listeria monocytogenes [Application Sheet C49]
Obligate intracellular bacteria [Application Sheet C49]
Piscirickettsia [Application Sheet C49]
Rickettsia typhi [Application Sheet C49]
Spore protoplast density determination [Application Sheet C49]
Soil, stream-water, clinical specimens and food [Application Sheet C39]

Cells in suspension, maintenance of [Application Sheet C38]
Erythrocytes [Application Sheet C34]

Cryptosporidium (see “Protozoa”)
Cyclospora (see “Protozoa”)

Dendritic cells
Barrier flotation [Application Sheet C21]
Barrier sedimentation [Application Sheet C41]
Mixer flotation [Application Sheet C22]

Enterocyozoon bieneusi (see “Protozoa”)

Erythrocytes (normal and sickle cells) [Application Sheet C34]

Erythrocytes, removal from blood and bone marrow [Application Sheet C34]

Foam cells [Application Sheet C42]

Gastric cells
Parietal cells [Application Sheet C48]
ECL cells [Application Sheet C47]

Granulocytes (see “Polymorphonuclear leukocytes”)

Hepatic cells
Non-parenchymal cells – a short methodological survey [Application Sheet C25]
Kupffer cells [Application Sheet C28]
Non-parenchymal cells [Application Sheet C26]
Stellate cells [Application Sheet C27]

Kidney cells (see “Renal”)

Langerhans cells (see “Dendritic cells”)

Lymphocytes
Blood and tissues, from (see “Mononuclear cells”)

Macrophages [Application Sheet C42]

Mattesia orzaephili (see “Protozoa”)

Microfluidic cell encapsulation/cell sorting [Application Sheet C38]

Monocytes (human)
Leukocyte-rich plasma
Barrier sedimentation [Application Sheet C46]
Flotation [Application Sheet C10]
Methodological review [Application Sheet C03]
Whole blood, from [Application Sheet C11]

Mononuclear cells
Bone marrow [Application Sheet C40]
Equine peripheral blood [Application Sheet C09]
Human peripheral blood
Barrier flotation [Application Sheet C06]
Barrier sedimentation [Application Sheet C04]
Mixer flotation [Application Sheet C05]
Intestine [Application Sheet C40]
Liver [Application Sheet C40]
Methodological review [Application Sheet C03]
Mouse blood
Barrier sedimentation [Application Sheet C43]
Mixer flotation [Application Sheet C08]
Non-human primate peripheral blood [Application Sheet C45]
Peritoneal exudates [Application Sheet C20]
Rabbit blood [Application Sheet C43]
Rat blood
Barrier sedimentation [Application Sheet C43]
Mixer flotation [Application Sheet C07]
Ruminant peripheral blood [Application Sheet C09]
Spinal cord [Application Sheet C40]
Spleen [Application Sheet C40]

Neural cells
Inflammatory cells (spinal cord injury) [Application Sheet C35]
Microglial cells [Application Sheet C35]
Motoneurons (brain, various sites) [Application Sheet C36]
Motoneurons (spinal cord) [Application Sheet C23]
Oligodendrocytes (see “Microglial cells”)

Neutrophils
see “Polymorphonuclear leukocytes”
Pancreatic islets [Application Sheet C16]
Pancreatic stellate cells [Application Sheet C27]
Plant protoplasts [Application Sheet C19]
Platelets (human) [Application Sheet C13]

Polymorphonuclear leukocytes
Human peripheral blood [Application Sheet C12]
Mouse blood [Application Sheet C44]
Non-human primate peripheral blood (see “Human
peripheral blood”)
Peritoneal exudates [Application Sheet C20]
Rat blood (see “ Mouse blood”)
Rabbit blood (see “ Mouse blood”)
Spinal injury, quantitative assessment in [Application Sheet C35]

Progenitor cells (bone marrow and other tissues)
Barrier sedimentation [Application Sheet C24]

Protozoa
Cryptosporidium [Application Sheet C31]
Cyclospora [Application Sheet C31]
Enterocytozoon bieneusi [Application Sheet C31]
Mattesia orzaephili [Application Sheet C31]
Plasmodium [Application Sheet C32]
Sarcocystis neurona [Application Sheet C31]
Tetrahymena thermophila [Application Sheet C31]
Toxoplasma
Purification from cell cultures [Application Sheet C33]
From soil samples [Application Sheet C33]
Separation of sporocysts and oocyst walls [Application Sheet C33]

Pulmonary cells
Endothelial cells [Application Sheet C29]
Epithelial cells (Type I) [Application Sheet C29]
Epithelial cells (Type II) [Application Sheet C30]
Lymphoid cells [Application Sheet C30]
Macrophages [Application Sheet C29]
Myeloid cells [Application Sheet C30]

Renal cells
Interstitial and thin loop of Henlé cells [Application Sheet C37]
Proximal tubule [Application Sheet C37]

Reticulocytes [Application Sheet C34]

Sarcocystis neurona (see “Protozoa”)

Sea urchin coelomocytes [Application Sheet C50]

Sickle cells (see “Erythrocytes”)

Sperm cells
Bovine [Application Sheet C17]
Equine [Application Sheet C17]
Elephant [Application Sheet C17]
Gazelle [Application Sheet C17]
Human [Application Sheet C18]
Mouse [Application Sheet C17]
Porcine [Application Sheet C17]
Turkey/rooster [Application Sheet C17]

Spleen
Dendritic cells (see “Dendritic cells”)
Mononuclear cells (see “Mononuclear cells”)
Splenocytes (see “Mononuclear cells”)

Stem cells (see “Progenitor cells”)

Thrombocytes (see “Platelets”)

Thymus
Dendritic cells (see “Dendritic cells”)

Viable/non-viable cells [Application Sheet C14]