Optiprep用于细胞分离的实验方案

发布:2022-02-17  更新:2024-01-14

OPTIPREP™ APPLICATION SHEET INDEX
EUKARYOTIC AND PROKARYOTIC CELLS

A. General methods for preparing and analysing gradients
B. An alphabetical cell type index. Commonly isolated cells such as mononuclear cells are often further categorized into species or tissue source. If a cell type is not in this index, it may be necessary to develop a customized method: in Section A see “Fractionation of a mixed population of cells” for some guidance.

A. GENERAL METHODOLOGY

Preparation of gradient solutions [Application Sheet C01]
Preparation of gradients [Application Sheet C02]
Fractionation of a mixed population of cells [Application Sheet C15]
Analysis of gradients [Application Sheet C51]

B. ALPHABETICAL CELL INDEX

Alveolar cells (see “Pulmonary cells”)
Bacteria
Anaplasma phagocytophilum [Application Sheet C49]
Chlamydophila abortus [Application Sheet C49]
Cyanobacteria [Application Sheet C49]
Listeria monocytogenes [Application Sheet C49]
Obligate intracellular bacteria [Application Sheet C49]
Piscirickettsia [Application Sheet C49]
Rickettsia typhi [Application Sheet C49]
Spore protoplast density determination [Application Sheet C49]
Soil, stream-water, clinical specimens and food [Application Sheet C39]

Cells in suspension, maintenance of [Application Sheet C38]
Erythrocytes [Application Sheet C34]

Cryptosporidium (see “Protozoa”)
Cyclospora (see “Protozoa”)

Dendritic cells
Barrier flotation [Application Sheet C21]
Barrier sedimentation [Application Sheet C41]
Mixer flotation [Application Sheet C22]

Enterocyozoon bieneusi (see “Protozoa”)

Erythrocytes (normal and sickle cells) [Application Sheet C34]

Erythrocytes, removal from blood and bone marrow [Application Sheet C34]

Foam cells [Application Sheet C42]

Gastric cells
Parietal cells [Application Sheet C48]
ECL cells [Application Sheet C47]

Granulocytes (see “Polymorphonuclear leukocytes”)

Hepatic cells
Non-parenchymal cells – a short methodological survey [Application Sheet C25]
Kupffer cells [Application Sheet C28]
Non-parenchymal cells [Application Sheet C26]
Stellate cells [Application Sheet C27]

Kidney cells (see “Renal”)

Langerhans cells (see “Dendritic cells”)

Lymphocytes
Blood and tissues, from (see “Mononuclear cells”)

Macrophages [Application Sheet C42]

Mattesia orzaephili (see “Protozoa”)

Microfluidic cell encapsulation/cell sorting [Application Sheet C38]

Monocytes (human)
Leukocyte-rich plasma
Barrier sedimentation [Application Sheet C46]
Flotation [Application Sheet C10]
Methodological review [Application Sheet C03]
Whole blood, from [Application Sheet C11]

Mononuclear cells
Bone marrow [Application Sheet C40]
Equine peripheral blood [Application Sheet C09]
Human peripheral blood
Barrier flotation [Application Sheet C06]
Barrier sedimentation [Application Sheet C04]
Mixer flotation [Application Sheet C05]
Intestine [Application Sheet C40]
Liver [Application Sheet C40]
Methodological review [Application Sheet C03]
Mouse blood
Barrier sedimentation [Application Sheet C43]
Mixer flotation [Application Sheet C08]
Non-human primate peripheral blood [Application Sheet C45]
Peritoneal exudates [Application Sheet C20]
Rabbit blood [Application Sheet C43]
Rat blood
Barrier sedimentation [Application Sheet C43]
Mixer flotation [Application Sheet C07]
Ruminant peripheral blood [Application Sheet C09]
Spinal cord [Application Sheet C40]
Spleen [Application Sheet C40]

Neural cells
Inflammatory cells (spinal cord injury) [Application Sheet C35]
Microglial cells [Application Sheet C35]
Motoneurons (brain, various sites) [Application Sheet C36]
Motoneurons (spinal cord) [Application Sheet C23]
Oligodendrocytes (see “Microglial cells”)

Neutrophils
see “Polymorphonuclear leukocytes”
Pancreatic islets [Application Sheet C16]
Pancreatic stellate cells [Application Sheet C27]
Plant protoplasts [Application Sheet C19]
Platelets (human) [Application Sheet C13]

Polymorphonuclear leukocytes
Human peripheral blood [Application Sheet C12]
Mouse blood [Application Sheet C44]
Non-human primate peripheral blood (see “Human
peripheral blood”)
Peritoneal exudates [Application Sheet C20]
Rat blood (see “ Mouse blood”)
Rabbit blood (see “ Mouse blood”)
Spinal injury, quantitative assessment in [Application Sheet C35]

Progenitor cells (bone marrow and other tissues)
Barrier sedimentation [Application Sheet C24]

Protozoa
Cryptosporidium [Application Sheet C31]
Cyclospora [Application Sheet C31]
Enterocytozoon bieneusi [Application Sheet C31]
Mattesia orzaephili [Application Sheet C31]
Plasmodium [Application Sheet C32]
Sarcocystis neurona [Application Sheet C31]
Tetrahymena thermophila [Application Sheet C31]
Toxoplasma
Purification from cell cultures [Application Sheet C33]
From soil samples [Application Sheet C33]
Separation of sporocysts and oocyst walls [Application Sheet C33]

Pulmonary cells
Endothelial cells [Application Sheet C29]
Epithelial cells (Type I) [Application Sheet C29]
Epithelial cells (Type II) [Application Sheet C30]
Lymphoid cells [Application Sheet C30]
Macrophages [Application Sheet C29]
Myeloid cells [Application Sheet C30]

Renal cells
Interstitial and thin loop of Henlé cells [Application Sheet C37]
Proximal tubule [Application Sheet C37]

Reticulocytes [Application Sheet C34]

Sarcocystis neurona (see “Protozoa”)

Sea urchin coelomocytes [Application Sheet C50]

Sickle cells (see “Erythrocytes”)

Sperm cells
Bovine [Application Sheet C17]
Equine [Application Sheet C17]
Elephant [Application Sheet C17]
Gazelle [Application Sheet C17]
Human [Application Sheet C18]
Mouse [Application Sheet C17]
Porcine [Application Sheet C17]
Turkey/rooster [Application Sheet C17]

Spleen
Dendritic cells (see “Dendritic cells”)
Mononuclear cells (see “Mononuclear cells”)
Splenocytes (see “Mononuclear cells”)

Stem cells (see “Progenitor cells”)

Thrombocytes (see “Platelets”)

Thymus
Dendritic cells (see “Dendritic cells”)

Viable/non-viable cells [Application Sheet C14]