加拿大CEDARLANE密度梯度分离液山进生物

CEDARLANE是加拿大一家公司，其密度梯度分离液产品有较多用户在用，其分离液产品的主要特点是拥有比其它公司更为细分的产品，除了常用的人淋巴细胞分离液和人血液多形核细胞分离液以外，还有专门针对小鼠、大鼠、兔子、哺乳动物等的淋巴细胞分离液。以下是该公司所有密度梯度分离液产品的列表。

货号	产品名称	包装规格	货期
CL5010	Lympholyte-H人淋巴细胞分离液	5 x 30 ml	2-3周
CL5015		100 ml	2-3周
CL5016		6 x 100 ml	2-3周
CL5020		500 ml	现货
CL5026		6 x 500 ml	现货
CL5030	Lympholyte-M小鼠淋巴细胞分离液	5 x 30 ml	2-3周
CL5031		100 ml	2-3周
CL5035		500 ml	2-3周
CL5040	Lympholyte-Rat大鼠淋巴细胞分离液	5 x 30 ml	2-3周
CL5041		100 ml	2-3周
CL5045		500 ml	2-3周
CL5050	Lympholyte-Rabbit兔淋巴细胞分离液	5 x 30 ml	2-3周
CL5055	Lympholyte-Rabbit兔淋巴细胞分离液	500ml	2-3周
CL5070	Lympholyte-poly多型核细胞分离液	100 ml	2-3周
CL5071	Lympholyte-poly多型核细胞分离液	250 ml	停产
CL6255	Lympholyte-1.1（the isolation of islet cells from the pancreas）	500 ml	现货
CL5110	Lympholyte-Mammal哺乳动物淋巴细胞分离液	5 x 30 ml	2-3周
CL5115		100 ml	2-3周
CL5120		500 ml	2-3周

常见问题：

Is there an expiry date for your Lympholyte® products?
We do not put an expiry date on most of our Lympholyte® products as they are stable for years when stored as recommended (i.e. unopened and protected from light). We have performed stability testing on Lympholyte® that is 5 years old, and found it to work very well.
I have Lympholyte® that is more than 5 years old. Is it OK to use?
If it has not been opened and has been stored properly (i.e. in the dark) it should work; however, it is best to use a recently produced batch.
You have several different Lympholyte® formulations. How do I know which one to use?
Which Lympholyte® you would use will depend on the species from which the lymphocytes are being isolated from, and whether they are being isolated from lymphoid organs or blood. We offer Lympholyte® specific for mouse, rat, rabbit and human lymphocytes (Lympholyte®-M, -R, Rabbit and -H, respectively). If you need to isolate lymphocytes from mammalian blood, we would recommend using Lympholyte®-Mammal or Lympholyte®-H. We also offer Lympholyte®-Poly for the isolation for human polymorphonuclear cells. For the isolation of cells whose density falls outside the range of the premade Lympholyte media, we offer Lympholyte®-1.1, a density gradient medium whose density can be set where needed.
Can I use Lympholyte®-M with peripheral blood samples, in addition to cells isolated from mouse tissue?
We recommend using Lympholyte®-Mammal with mouse blood, however you can use Lympholyte®-M as well. You will not recover as many cells as you would using Lympholyte®-Mammal, and there will be more RBC contamination in the interface.
Why do I have to use a serum-free media when using Lympholyte® for cell isolation?
Serum may cause the cells the clump together which causes them to pellet, leading to lower lymphocyte recovery.
Can I isolate granulocytes using Lympholyte®?
Lympholyte®-Poly is designed for isolating human granulocytes. Unfortunately, none of the other Lympholyte® products will do this, and the granulocytes end up in the pellet at the bottom of the tube. By using a RBC lysing buffer (i.e. NH4Cl) and some washing steps, you can purify your granulocytes from the pellet. For more information, please contact our technical services department.